(P-425) Intracytoplasmic sperm injection induces transgenerational abnormalities in mice.

Abstract Authors:


Mito Kanatsu-Shinohara,^1,5 Yusuke Shiromoto,^1,5 Narumi Ogonuki,² Kimiko Inoue,² Satoko Hattori,³ Kento Miura,² Naomi Watanabe,² Ayumi Hasegawa,² Keiji Mochida,² Takuya Yamamoto,⁴ Tsuyoshi Miyakawa,³ Atsuo Ogura,² and Takashi Shinohara¹





1 Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
2 Bioresource Engineering Division, RIKEN BioResource Research Center, Ibaraki, Japan.
3 Division of Systems Medical Science, Center for Medical Science, Fujita Health University, Toyoake, Japan.
4 Department of Life Science Frontiers, Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan.
5 These authors contributed equally.













Abstract Text: In vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) are 2 major assisted reproductive techniques (ARTs) used widely to treat infertility. Recently, spermatogonial transplantation emerged as a new ART to restore fertility to young patients with cancer after cancer therapy. To examine the influence of germ cell manipulation on behavior of offspring, we produced F₁ offspring by a combination of two ARTs, spermatogonial transplantation and ICSI. When these animals were compared with F₁ offspring produced by ICSI using fresh wild-type sperm, not only spermatogonial transplantation–ICSI mice but also ICSI-only control mice exhibited behavioral abnormalities, which persisted in the F₂ generation. Furthermore, although these F₁ offspring appeared normal, F₂ offspring produced by IVF using F₁ sperm and wild-type oocytes showed various types of congenital abnormalities, including anophthalmia, hydrocephalus, and missing limbs. Therefore, ARTs can induce morphological and functional defects in mice, some of which become evident only after germline transmission.