Graduate Student University of Illinois Urbana-Champaign Champaign, Illinois, United States
Abstract Authors: Audrey Fields1, Andressa Varella Gonsioroski2, and Jodi Flaws1
Department of Comparative Biosciences and Division of Nutritional Sciences
D 1. Department of Comparative Biosciences, University of Illinois at Urbana-Champaign, Urbana, IL
< 2. Veterinary Diagnostic Laboratory, Pathobiology and Diagnostic Investigation Department, Michigan State University
Abstract Text: The incorporation of water disinfectants into the main water supply has significantly decreased the presence of waterborne diseases such as cholera. However, the interaction between disinfectants and organic material generates water disinfection byproducts (DBPs). Iodoacetic Acid (IAA) is one unregulated DBP and has been reported as a reproductive toxicant. Our previous studies show that IAA inhibits follicle growth and alters the expression of steroidogenic factors in vitro, but its effects on the expression of oxidative stress markers are unknown. Thus, this study tested the hypothesis that IAA exposure alters gene expression of oxidative stress markers in the mouse ovary. To test this hypothesis, adult CD-1 mice were dosed with either water (control) or IAA (0.5 mg/L; 10 mg/L; 100 mg/L; 500 mg/L) in their drinking water for 35 days. Following this, ovaries were collected when the mice were in diestrus, RNA was extracted, reverse transcribed, and subjected to quantitative polymerase chain reaction (qPCR) to analyze the expression of oxidative stress markers with glutathione properties (Gstt1, Gstp1, Gsta1, Gsto1), antioxidant properties (Gpx1, Gsr, Cat), and superoxide properties (Sod1, Sod2). IAA exposure decreased expression of glutathione oxidative stress markers Gstt1 for the 0.5 mg/L, 10 mg/L and 500 mg/L treatment groups compared to the control and Gstp1 for the 10 mg/L treatment group compared to the control, but did not affect the expression of Gsto1 or Gsta1 compared to control. Further, IAA exposure decreased the expression of the antioxidant oxidative stress marker Cat for the 0.5 gm/L, 10 mg/L, 100 mg/L and 500 mg/L IAA treatment groups compared to the control, but did not affect the expression of Gxp1 or Gsr compared to control. In addition, IAA significantly decreased the expression of the superoxide oxidative stress marker Sod1 for the 0.5 gm/L, 10 mg/L and 100 mg/L IAA treatment groups compared to the control, but did not affect the expression of Sod2 compared to control. These data indicate that IAA exposure alters oxidative stress gene expression in the mouse ovary. This project is supported by NIH R21ESO28963.
