Professor Sungshin University Seongbuk-Gu, United States
Abstract Authors: Bo Young Lee, Yong-Pil Cheon
Division of Developmental Biology and Physiology, Department of Biotechnology, Institute for Basic Sciences, Sungshin University, Seoul 02844, Korea
Abstract Text: Increased primordial follicle activation induces immoderate depletion of ovarian reserve and premature ovarian insufficiency, so the regulation of primordial follicle activation is one of the crucial steps. Steroid hormones, especially estrogen, are known to play an important role in relation to nest breakdown and subsequent follicle development. Although a relationship between the primordial follicle activation and E2 concentration is predicted in many studies, it is not well evaluated. In this study, the anti-Müllerian hormone (Amh) knockout mice and in vitro culture were employed to treat specific antagonist and agonist of the estrogen receptors and to compare the degree of activation. As expected, the delivery rate of Amh null mice by aging was significantly decreased but the level of phosphate Akt was significantly high. Interestingly the expression levels of Amh mRNA was significantly low but the protein level was significantly highi in the ovary of Amh null mice. The expression levels of the Esr1 mRNA and protein were significantly low in Amh KO ovary but not Esr2 and Gper1. In some follicles, ESR1 and FOXO3 were colocalized in nucleus. The expression of genes could be clustered by the culture periods. Although further studies are needed, the results suggest that Esr may have roles in follicle dormancy.
Grant: This study was supported by the NRF-2021K1A4A7A02098794 and NRF-2023R1A2C3007223
