Postdoctoral researcher University of Sassari SASSARI, Sardegna, Italy
Abstract Authors: Mohammadreza Ebrahimi1; Laura Mara2; Sara Succu1; Sergio Domenico Gadau1; Fabrizio Chessa2; Maria Dattena2; Alberto Maria Luciano3; Fiammetta Berlinguer1
1. Department of Veterinary Medicine, University of Sassari, Via Vienna 2, Sassari, Italy
2. Department of Animal Science, Agricultural Research Agency of Sardinia, 07100 Sassari, Italy
3. Reproductive and Developmental Biology Laboratory (ReDBioLab), Department of Veterinary Medicine and Animal Sciences, University of Milan, Via dell’Università, 6 - 26900 Lodi, Italy
Abstract Text: Long in vitro culture (LIVC) of oocytes derived from early antral follicles (EAFs) has emerged as a potential reproductive technology for producing large numbers of competent oocytes. In a previous study, we demonstrated that LIVC of cumulus-oocyte complexes (COCs) from EAFs supports oocyte survival, growth, and acquisition of meiotic competence in adult sheep. This promising approach offers opportunities for preserving valuable and endangered animals, shortening generation intervals, and increasing genetic gain, especially when ovaries are collected from prepubertal animals as a source of EAFs. With this in mind, our objective was to investigate the efficacy of LIVC of COCs collected from EAFs in lambs. To achieve this, lamb ovaries were collected, and COCs were retrieved from EAFs (350-450 µm) by rupturing the follicle wall using a 21-gauge needle. Subsequently, they were cultured in a 96-well plate (one COC per well) containing TCM199 supplemented with 0.15 μg/mL zinc sulfate, 10-4 IU/mL FSH, 10 ng/mL estradiol, 50 ng/mL testosterone, 50 ng/mL progesterone, and 5 μM Cilostamide. After 5 days of culture, the following parameters were evaluated: COC morphology, oocyte diameter, chromatin configuration, gap junction communications, meiotic competence, levels of reactive oxygen species (ROS), mitochondrial activity, and distribution. Following the LIVC process, the results indicate a significant increase in oocyte diameter (108.76 vs. 113.44 µm, p< 0.000) and in mitochondrial activity (p< 0.005), with a change in mitochondrial distribution pattern from 'fine' to 'granular' following the LIVC and IVM process (p< 0.01). Furthermore, a notable transition in chromatin configuration was observed, characterized by a shift from a non-surrounded nucleolus (NSN) to a surrounding nuclear envelope (SNE) stage (p< 0.000), coupled with spontaneous meiosis resumption after LIVC (MI/MII, 26.7%), and an increase in meiosis resumption after IVM (p< 0.000). However, these results were accompanied by a significant increase in low quality COCs (based on morphological evaluation) (p< 0.05) and an increase in ROS levels (p< 0.000). Most COCs also exhibited closed gap junction communications before and after LIVC (p< 0.01), indicating a defective cell coupling between oocytes and cumulus cells.
